Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells? Transcribed Image Text: Spread-plate method
Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells? Transcribed Image Text: Spread-plate method
Solid agar
Incubate
Culture, diluted
as needed
Bacterial colonies
0.1-0.2 mL
appear only on surface.
Spread cells onto surface
of pre-poured solid agar.
Pour-plate method
0.1-1.0 mL
Melted cooled agar
Incubate
FIGURE 4.18 Spread Plates and Pour Plates
Some colonies appear on
surface; many are below surface.
These techniques can be used for plate counts.
Add melted cooled agar
and swirl gently to mix.

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